Figure 10.

Blood CD1c⁺ dendritic cells (DCs) but not monocytes are a source of interleukin (IL)-12p40 in response to Leishmania major. Human blood CD1c⁺ DCs, CD14⁺ monocytes and NKp46⁺CD3⁻ natural killer (NK) cells were sorted and stimulated by L. major promastigotes (multiplicity of infection 10) for 20 h either alone or in combinations. R848 (5 µg/ml) was used as control. IL-12p40 content of the cell culture supernatants was measured by ELISA. Mean ± SEM of 4/4/3 donors for the three stimulations.