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. 2018 Jan 24;9:24. doi: 10.3389/fimmu.2018.00024

Figure 7.

Figure 7

CD69 upregulation on natural killer (NK) cells by supernatants (SNs) of peripheral blood mononuclear cell (PBMC)/Leishmania cocultures is maintained after neutralization of NK cell-activating cytokines. Human PBMCs were stimulated with different (A) recombinant cytokines or (A,B) SNs of PBMC/Leishmania cocultures of the respective donors for 20 h, followed by analysis of CD69 surface expression on NKp46+CD3− NK cells by flow cytometry. (B) Before stimulation, the cytokines or SNs were pretreated with either one or several neutralizing antibodies (Abs) (37°C, 1–2 h). The concentrations of the cytokines and Abs were as described in Section “Materials and Methods.” (A) Mean ± SEM of 57/57/17/11/12/8/19/8/7/3/3 donors for the 11 stimulations. (B) Mean ± SEM of 7/7/7/6/7/7/7 [SN + anti-interferon (IFN)-α/β, anti-interleukin (IL)-12, and anti-IL-18], 7/7/7/7/6/7 (SN + anti-IL-1β), 9/9/9/7/8/9 (SN + anti-IL-15), 5/5/5/5/5/5 (SN + anti-IL-2), or 4/4/4/4/4/4 (SN + anti-IL-6) donors for the different stimulations. #p < 0.05, ##p < 0.01, and ###p < 0.001 two-tailed Mann–Whitney U test.