FIGURE 2.

Time lapse images of live E. coli showing changes to FtsZ-GFP and GFP-FtsA localization imaged with LSCM (GFP 488/509 nm). In the absence of 2,4-D, E. coli showed FtsZ forming a centrally localized Z-ring (A–C), but upon addition of 1 mM 2,4-D in the imaging medium, FtsZ dissociated from the Z-ring within 5 s forming bright spots (D–F), mostly near the periphery and poles. GFP-FtsA also showed a similar change in localization (G–L). The 1 mM 2,4-D was added to the imaging medium at 10 s. Valinomycin (30 μM) (M–P) and nigericin dissolved in DMSO (10 μM) (Q–T) also caused delocalization of FtsZ and FtsA, and DMSO control had no effects on FtsZ (M,Q) and FtsA (O,S).