FIGURE 3.

Images showing the location of the Z-ring and nucleoid after long term exposure to 2,4-D. In the absence of 2,4-D (A–C) E. coli showed a centrally localized Z-ring (B, arrow) with two nucleoids on either side (C, arrow). Exposure to 4 mM 2,4-D resulted in elongated cells with multiple partial Z-rings along the axis (E, arrow) and a large nucleoid mass (F, arrow) after an overnight exposure (D–F). After 3 h exposure (G–I), the majority of the cells lacked a typical Z-ring (H, arrow). The first panel shows the GFP+DAPI overlay, the second panel is only FtsZ-GFP and the third shows only the DAPI stain on the nucleoid.