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. Author manuscript; available in PMC: 2018 Feb 13.
Published in final edited form as: Biochem J. 2016 Apr 12;473(12):1759–1768. doi: 10.1042/BCJ20160270

Figure 3. CARD14E138A mutation activates MALT1 paracaspase activity.

Figure 3

(A) HEK293 cells were co-transfected with vectors encoding V5-CARD14 variants (0.1–0.4 μg), WT or C464 FLAG-MALT1 (0.8–1.2 μg) and HA-BCL10 (0.8–1.4 μg). Active MALT1 was assayed by ABP pull down and immunoblotting. Representative of three independent experiments. (B) HaCaT keratinocytes were co-transfected with FLAG-CARD14 variant (0.125–0.2 μg) or EV, pNFκB-Luc and pRL-TK vectors. 5 h after transfection, cells were treated with mepazine or DMSO vehicle and lysed after further 24 h culture. NF-κB activity was determined as in Figure 1(D) (mean ± S.E.M. of three independent experiments). Representative expression of FLAG-CARD14 was determined by immunoblotting of corresponding lysates. Significance determined by two-way ANOVA.