Fig. 6.

Time-resolved glycerol secretion from an adipose tissue explant using 3D-templated reservoirs and a 3D-printed tissue trap on a microfluidic device under continuous, vacuum-driven flow. After switching from high insulin and glucose (2 nM insulin, 19 mM glucose) to low insulin and glucose (50 pM insulin, 3.5 mM glucose) solutions at the 30 min time point, glycerol secretion rates increased. Average data is shown for three explants, with error bars representing standard deviations