Effect of CB1/CB2 double knockout on infarct volume, infarct fraction, clinical score, and regional cerebral blood flow in a mouse model of transient ischemia/reperfusion (MCAO). A: X axis: genotype, Y axis, infarct volume in mm3. Double knockout (DKO) are not significantly different compared to wild type controls. B: X axis: genotype, Y axis, infarct fraction as a percent of the whole ipsilateral hemisphere. DKO mice are not significantly different as compared to wild type controls (p = 0.0014). C: X axis: genotype, Y axis, outcome of the neurological assessment (a higher score indicates more severe impairment). DKO mice show significantly improved neurological performance compared to wild type controls (p = 0.008). D: X axis, time following middle cerebral artery occlusion, Y axis, % baseline flow measured in laser Doppler units. Double knockout (DKO) mice show significantly improved neurological performance compared to wild type controls (two way ANOVA significant main effect of genotype F= 36.25, p < 0.0001; Sidak’s multiple comparison test shows significance within the second 5 minutes of occlusion * p<0.05)).