Figure 4.

Morphological neoplastic transformation induced by silver nanoparticles (AgNPs) in Balb/c 3T3 A31-1-1 cells. (A) Number of foci per plate, (B) transformation results (Tf). Cells were seeded onto a 100-mm dish and treated with AgNPs (0.17, 0.66, 2.65, 5.30, or 10.60 μg/mL), benzo(a) pyrene (B[a]P, 1 μg/mL), or anthracene (An, 20 μg/mL). Transformation frequency (Tf) was calculated as indicated in the materials and methods section. Results are expressed as the mean±standard deviation. **p<0.01.