Figure 9.

Differential expression of miRNAs and predicted target mRNAs in infected Bt-resistant and susceptible G. mellonella larvae. The miRNAs identified by microarray analysis and their predicted mRNA targets were validated by RT-PCR to confirm differential expression in infected resistant and susceptible G. mellonella larvae: (A) dvi-miR-964–5p; (B) contigs 19930 and 02874. The relative fold differences indicated for the miRNAs and mRNAs are normalized against bmo-miR-184–5p and 18S rRNA as the internal reference control. (* p < 0.05– fold expression in the fat body and midgut of infected susceptible line and infected resistant line were compared with fold expression of uninfected susceptible line and uninfected resistant line for the same organ respectively).