Figure 6.

Immunophenotyping of cells infiltrating the lungs shows increased levels of monocytes and macrophages in S. pneumoniae infected mice. Flow cytometry was used to characterize the immune cells recruited to the lungs of S. pneumoniae infected mice and uninfected wildtype (WT) and Muc1^−/− mice (n = 5–6). There were significantly more alveolar macrophages, interstitial macrophages and Ly6c- monocytes in the lungs of infected Muc1^−/− mice as compared with uninfected Muc1^−/− mice. For WT mice, there were significantly more alveolar macrophages in infected mice compared with uninfected mice (*p < 0.05, **p < 0.01, Student's t-test).Graphs present the median (horizontal bar), interquartile range (box) and 10th and 90th percentiles (bars). DC-dendritic cells; Uninf, uninfected; Inf, infected.