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. 2017 Jul 13;8(8):1631–1642. doi: 10.1080/21505594.2017.1341021

Figure 7.

Figure 7.

MUC1 facilitates the phagocytosis and killing of S. pneumoniae. Macrophages from wildtype (WT) and Muc1−/− mice (n = 5) were co-cultured in vitro with CFSE-labeled S. pneumoniae strain D39, TIGR4A, PMP1 and PMP1287 and P. aeruginosa strain NCTC10662 (multiplicity of infection 50:1) for time-points shown. (A) Muc1−/− macrophages have significantly decreased intracellular S. pneumoniae D39, TIGR4A and PMP1278 strains as compared with WT cells (*p < 0.05, **p < 0.01, Student's t-test). (B) Significantly reduced killing of S. pneumoniae D39 strain by Muc1−/− macrophages compared with WT macrophages was observed after 120 min of co-culture (**p < 0.01, Student's t-test). (C) While intracellular S. pneumoniae was significantly higher in WT macrophages as compared with Muc1−/− macrophages, Muc1−/− macrophages had significantly increased phagocytosis of P. aeruginosa 60 mins after co-culture (**p < 0.01, ***p < 0.001, Student's t-test). Data shown are representative of 4 experiments for S. pneumoniae D39 and 1 for other bacterial strains. Graphs present the median (horizontal bar), interquartile range (box) and 10th and 90th percentiles (bars).