Table 1.
Candida albicans mutant libraries constructed from different background strains.
| Librarya | Method | Background strainb | Strains generated | Strains responding to treatment | Genes analyzed | Study (ref.) | Target(s) of the studyc |
|---|---|---|---|---|---|---|---|
| Heterozygous insertion | Tagged Tn5-UAU1 | BWP17 | 3,633 | 487 | 269 | Oh (22) | Identification of fitness genes and potential drug targets. |
| Protein kinase | Tn7-UAU1 | BWP17 | 108 | 80 | 80 | Blankenship (19) | Characterization of cell wall PK network. |
| Haploinsufficient | Tn7-URA3 | CAI4 | 18,000 | 325 | 146 | Uhl (16) | Identification of genes affecting yeast-hypha transition. |
| Homozygous insertion | Tn7-UAU1 | CAI4 | 353 | 3 | 3 | Davis (17) | Identification of genes affecting pH-dependent filamentation. |
| Homozygous insertion | Tn7-UAU1 | BWP17 | 197 | 30 | 30 | Finkel (20) | Characterization of the C. albicans cell surface regulatory networks. |
| TR homozygous insertion | Tn7-UAU1 | DAY185 | 83 | 2 | 2 | Nobile (65) | Identification of biofilm development regulatory proteins. |
| GRACE | Conditional expression | CaSS1 | 1,152 | 574 | 567 | Roemer (24) | Systematic discovery of essential genes and prioritization as antifungal targets. |
| GRACE | Conditional expression | Not reported | 2,356 | 974 | 974 | O'Meara (25) | Identification of TRs governing morphogenesis in response to host-relevant cues. |
| Heterozygous gene deletion | Gene replacement with HIS3 | Not reported | 2,868 | 84 | 69 | Xu (26) | Identification of genes related to the MOA, and the MOA of novel antifungal agents. |
| Homozygous gene deletion | Multi-marker gene-knockout | SN152 | ∼3000 | 115 | 115 | Noble (28) | Identification of genes required for infectivity in a mouse model and for morphological switching and cell proliferation in vitro. |
| TRKO homozygous gene deletion | Long-flanking-homology disruption | SN152 | 365 | 365 | 143 | Homann (27) | Phenotypic profiling of the C. albicans regulatory network. |