Figure 2.

TC-83 infection of C6/36 mosquito cells resulted in no significant loss of membrane potential or increase in reactive oxygen species. (A) TMRE fluorescence analysis of uninfected (mock) and TC-83 infected U87MG cells, and C6/36 mosquito cells indicating no increased mitochondrial damage in the C6/36 cell line. *indicates significance between U87MG infected and mock fluorescence. (B) Non-significant accumulation in ROS detected in infected C6/36 cells using DCFH-DA reagent. Data represents an average of two independent experiments performed with triplicate samples. (C) Extracellular viral titers as determined by plaque assay in U87MG and C6/36 cells.