Figure 4.

Supernatant of EAEC strain 042 has little effect on mortality. Strain 042 was grown stationary overnight in either LB-broth or DMEM/0.5 % glucose at 20 and 37 °C. The supernatants were then sterile filtered and concentrated 10 times using an 10 kDa amicon filter (Merck Millipore, Kenilworth, NJ), and 10 μl were injected into the larvae. The results represents the average of 3 experiments, repeated with 3 different batches of larvae. Statistical difference was calculated using the log rank test for multiple comparisons in GraphPad, however no statistical significance was observed.