Figure 3.
D. melanogaster Tep2 and Tep6 differentially regulate the activation of immune pathways against Photorhabdus. Transcript levels for (A, B) Defensin (Toll pathway), (C, D) Diptericin (IMD pathway), (E, F) Tot-M (JAK/STAT pathway) and (G, H) Puckered (JNK pathway) in loss-of function tep2 and tep6 mutants with their corresponding control strain (w1118) at 6 and 18 hpi with 1XPBS, E. coli (Ec), P. luminescens (Pl) or P. asymbiotica (Pa) (n = 3 individuals per experimental condition). Gene transcript levels are shown as relative abundance of transcripts normalized to RpL32 and expressed as a ratio compared with untreated flies (negative control). Values represent the means from 3 biologic replicates and error bars represent standard deviations. Significant differences are indicated with asterisks; *p <0.05, **p <0.01, ***p <0.001, ****p <0.0001).