Gene
|
Accession number
|
Primer
|
Primer Sequence
|
Tm (°C)
|
Mcf-1 |
BX571872 |
Forward |
5′-TTGGCGGGGTGGTAGTCG-3′ |
61 |
Reverse |
5′-CAGTTCAGCTTCCTTCTCTAA-3′ |
16s rRNA |
CP010445 |
Forward |
5′-GGAAGAAGCTTGCTTCTTTGCTGAC-3′ |
61 |
Reverse |
5′-AGCCCGGGGATTTCACATCTGACTTA-3′ |
TccC3 |
FM162591 |
Forward |
5′-CGGCAGCGGAATAAGTCAGAG-3′ |
61 |
Reverse |
5′-TCGATGGTCAAGAGGCAAACTG-3′ |
RpL32 |
CG7939 |
Forward |
5′-GATGACCATCCGCCCAGCA-3′ |
61 |
Reverse |
5′-CGGACCGACAGCTGCTTGGC-3′ |
Tep1 |
CG18096 |
Forward |
5′-AGTCCCATAAAGGCCGACTGA-3′ |
61 |
Reverse |
5′-CACCTGCATCAAAGCCATATTG-3′ |
Tep2 |
CG7052 |
Forward |
5′-TGTTCTGCACCAACAGCGATAC-3′ |
61 |
Reverse |
5′-CTGGCGATCCATCAACATTCTT-3′ |
Tep3 |
CG7068 |
Forward |
5′-TCCAAGGGTCCATGTGATGC-3′ |
61 |
Reverse |
5′-TAATCCCAACCCGTTCACCG-3′ |
Tep6 |
CG7586 |
Forward |
5′-CGCCTTCCTGAACGAAACAA-3′ |
61 |
Reverse |
5′-GAGGCTTATCGGTCTGCACAA-3′ |
Defensin |
CG1385 |
Forward |
5′-CGCATAGAAGCGAGCCACATG-3′ |
56 |
Reverse |
5′-GCAGTAGCCGCCTTTGAACC-3′ |
Diptericin |
CG10794 |
Forward |
5′-ACCGCAGTACCCACTCAATC-3′ |
57 |
Reverse |
5′-CCCAAGTGCTGTCCATATCC-3′ |
Turandot-M |
CG14027 |
Forward |
5′-GGTTTGCTTCAGCGTTCCAAAAAGTCATAACC-3′ |
61 |
Reverse |
5′-ATTAAAACAATATTAACCAGTGAATAATTGAG-3′ |
Puckered |
CG7850 |
Forward |
5′-GGCCTACAAGCTGGTGAAAG-3′ |
61 |
Reverse |
5′-AGTTCAGATTGGGCGAGATG-3′ |
Eater |
CG1624 |
Forward |
5′-ATAACGATCCATCTAACCGATGTGT-3′ |
57 |
Reverse |
5′-GATTGGCAGGTTCCTCGACTAC-3′ |