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N-linked mannosylation of Plb1 and secretome composition are altered in arg1Δ. Cryptococcal secretions, each containing 40 µg of protein, were collected from WT and arg1Δ, treated with PNGase F (+), resolved by SDS-PAGE and subjected to either (A) anti-Plb1 Western blotting, (B) glycoprotein staining or (C) glycoprotein staining followed by Commassie staining. In (C), the arrow indicates the position of the PNGase F enzyme.
