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Snail involved in TGF-β1-induced EMT Cells were treated with TGF-β1 in the absence or presence of OST co-treatment and the protein and mRNA expressions of Snail were determined by Western blotting (A and B) and qRT-PCR (C). Snail was silenced with siRNA (D) and then treated with TGF-β1 (5 ng/ml) for 48 h, the protein expression was determined by Western blotting. *p < 0.05, **p < 0.01. OST, osthole.
