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. 2018 Feb 9;11:757–767. doi: 10.2147/OTT.S155065

Figure 3.

Figure 3

Cardamonin downregulated the expression of PRAS40-S183.

Notes: SKOV3 cells were incubated with cardamonin (5 and 20 μM), rapamycin (0.1 μM), and AZD8055 (0.1 μM) for 2, 16, and 24 h. Western blotting was used to detect the expression of PRAS40-S183, PRAS40-T246, and total PRAS40. (A) Autoradiograph of expression of PRAS40-S183, PRAS40-T246, and total PRAS40. The density ratios of each band by PRAS40-S183/β-actin (B), PRAS40-T246/β-actin (C), and PRAS40/β-actin (D). n=3, #P<0.05 vs control group; *P<0.05 vs cardamonin (5 μM) group.