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. 2018 Feb 9;13:571–581. doi: 10.2147/COPD.S150633

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© 2018 Wang et al. This work is published and licensed by Dove Medical Press Limited

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GRP78 regulates CSE-induced inflammatory response and mucus production in HBE cells.

Notes: (A and B) HBE cells were exposed to 2% CSE at indicated times, and qRT-PCR was performed to assess the relative level of IL6 and IL8 mRNA transcripts. HBE cells were transfected with control or GRP78-siRNA for 24 h, the relative mRNA levels of GRP78 were measured (C) and then were treated with 2% CSE for an additional 24 h. The relative levels of IL6 (D), IL8 (E), and MUC5AC (H) mRNA transcripts were measured by qRT-PCR, and the protein levels of IL6 (F) and IL8 (G) in the culture supernatants were measured by ELISA. (I) Expression of MUC5AC was measured by Western blotting. Data (A–I) were mean ± SEM of three independent experiments. Western blots (I) were representative of three independent experiments. *P<0.05 and **P<0.01 (Student’s t-test).

Abbreviations: CSE, cigarette smoke extract; GRP78, glucose-regulated protein 78; HBE, human bronchial epithelial; IL, interleukin; qRT-PCR, quantitative reverse transcription polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay; SEM, standard error of the mean.

GRP78 regulates CSE-induced inflammatory response and mucus production in HBE cells.

Notes: (A and B) HBE cells were exposed to 2% CSE at indicated times, and qRT-PCR was performed to assess the relative level of IL6 and IL8 mRNA transcripts. HBE cells were transfected with control or GRP78-siRNA for 24 h, the relative mRNA levels of GRP78 were measured (C) and then were treated with 2% CSE for an additional 24 h. The relative levels of IL6 (D), IL8 (E), and MUC5AC (H) mRNA transcripts were measured by qRT-PCR, and the protein levels of IL6 (F) and IL8 (G) in the culture supernatants were measured by ELISA. (I) Expression of MUC5AC was measured by Western blotting. Data (A–I) were mean ± SEM of three independent experiments. Western blots (I) were representative of three independent experiments. *P<0.05 and **P<0.01 (Student’s t-test).

Abbreviations: CSE, cigarette smoke extract; GRP78, glucose-regulated protein 78; HBE, human bronchial epithelial; IL, interleukin; qRT-PCR, quantitative reverse transcription polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay; SEM, standard error of the mean.