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. 2018 Jan 17;30(1):134–152. doi: 10.1105/tpc.17.00626

Figure 2.

Figure 2.

Overexpression of HD2C Results in H4K16 Hypoacetylation in N. benthamiana.

(A) Schematic diagram of detecting HD2C-induced histone acetylation change in N. benthamiana.

(B) Immunostaining with H4K16ac antibody in nuclei overexpressing HD2C-GFP. Arrows represent the transfected nuclei expressing HD2C-GFP. Bar = 10 μm.

(C) Quantification of H4K16ac immunostaining performed with HD2C-GFP, HD2B-GFP, and HDA6-GFP. Numbers at the top represent the number of nuclei counted for each experiment.

(D) Quantification of immunostaining performed with HD2C-GFP with other histone acetylation antibodies. Numbers at the top represent the number of nuclei counted for each experiment.

(E) Detection of histone acetylation levels in hd2c mutant and HD2C overexpression (HD2C-GFP) plants by immunoblots. Numbers represent average band intensity of five technical immunoblot repeats (mean ± sd) normalized to H4.