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. 2017 Dec 14;30(1):209–227. doi: 10.1105/tpc.17.00778

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Figure 4. — RIP Assay for the Binding of APUM24 to 45S Pre-rRNA.

(A) Organization of 45S pre-rRNA and positions of specific primers used to amplify regions 1 to 8. Vertical lines indicate the site of endo- or exonuclease processing steps. ETS and ITS are external and internal transcribed spacer, respectively.

(B) RT-qPCR analysis of RNA coimmunoprecipitated with APUM24-FLAG protein by anti-FLAG antibodies. Enrichment of pre-rRNA or rRNA with specific regions (regions 1 to 8 in [A]) by coimmunoprecipitation was calculated with values obtained using the output sample versus those using the input sample. UBQ10 is shown as a non binding control. Error bars represent sd (n = 3). Asterisks indicate statistically significant differences between values obtained with apum24-2 (Control) and apum24-2 expressing APUM24-FLAG (APUM24) by Student’s t test (P < 0.05). The lower panel shows a view enlarged in the vertical direction.