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. 2017 Dec 14;30(1):209–227. doi: 10.1105/tpc.17.00778

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Figure 6. — Circular RT-PCR Analysis Revealed Overaccumulation of 27SB Pre-rRNA in the apum24-2 Mutant.

Products of cRT-PCR targeting RNA containing the 25S rRNA sequence (A) or 18S rRNA sequence (B) were analyzed by 2% agarose gel electrophoresis and the gels were stained with ethidium bromide. Three independent clones corresponding to ∼150- and 500-bp products in (A) were sequenced to confirm that the former and latter bands were derived from mature 25S rRNA and 27SB rRNA, respectively. Three independent clones corresponding to the ∼250-bp product in (B) were also sequenced to confirm that it was derived from mature 18S rRNA. M, DNA marker.