Figure 8.

Sugar Responses of apum24-2, brx1-1, and brx1-2.

(A) Relative expression levels of APUM24, BRX1-1, and BRX1-2 in 8-d-old seedlings treated with 50 mM mannitol (Man), fructose (Fru), glucose (Glc), or sucrose (Suc) for 3 h. The expression levels of these genes in untreated seedlings (0 h) were set to 1. The sugar-responsive marker gene, At1g80130 (Price et al., 2004), was used as a control, and UBQ10 was used as an internal control. Error bars represent sd (n = 3).

(B) Seedlings of the wild type, apum24-2, the complemented apum24-2 line (Complemented), brx1-1, and brx1-2 were grown for 7 d on 1/2 MS medium plates containing the indicated concentrations of glucose (Glc) or mannitol (Man). Bar = 1 mm.

(C) Relative anthocyanin levels in the seedlings in (B). The anthocyanin content of wild-type seedlings grown without sugar was set to 1. Error bars represent sd (n = 3). Statistical significance was determined by ANOVA, followed by a Tukey-Kramer test under each experimental condition. Means that are significantly different from each other (P < 0.05) are indicated by different letters.