The mlk1 mlk2 Double Mutants Are Hyposensitive to GAs.
(A) Cell lengths in hypocotyls of 1-week-old Col-0 and mlk1 mlk2 double mutant plants under LD. The cell sizes were marked with red lines.
(B) and (C) Cell length and cell number in 7-d-old Col-0 and mlk1 mlk2 double mutants under LD. Means ± sd obtained from over 40 independent plants.
(D) Hypocotyl lengths of Col-0, mlk1 mlk2 double mutants, and rga-28 grown on increasing concentrations of GA3 (0, 0.5, 1.0, 2.0, and 5.0 µM) under LD. Means ± sd obtained from over 40 independent plants.
(E) Relative responses of hypocotyl lengths in Col-0, mlk1 mlk2 double mutants, and rga-28 to GA3 treatment under LD. The response of Col-0 to GA3 is considered 100%, and the response of all mutants to GA3 is shown relative to Col-0. Means ± se; n = 3, where n is the number of independent experiments.
(F) Hypocotyl lengths of Col-0, mlk1 mlk2 double mutants, and rga-28 grown on increasing concentrations of PAC (0, 0.01, 0.02, 0.05, 0.1, 0.2, and 0.5 µM) under LD. Means ± sd obtained from over 40 independent plants.
(G) Relative responses of hypocotyl lengths in Col-0, mlk1 mlk2, and rga-28 to PAC treatment under LD. The response of Col-0 to PAC is considered 100%, and the response of all mutants to PAC is shown relative to Col-0. Means ± se; n = 3, where n is the number of independent experiments.