Figure 8. Expression of VCAM1 by the valvular endothelium.
Immunohistochemistry of the valvular endothelium in the context of acute rheumatic fever and valvulitis is shown. Vascular cell adhesion protein 1 (VCAM1) has been labelled with an VCAM1-specific monoclonal antibody (mAb) (red). The VCAM1-specifc mAb reacted with the rheumatic valvular endothelium but not with the normal valve (not shown). That is, the antibody to anti-VCAM1 is conjugated to alkaline phosphatase and developed with fast red indicated binding of the antibody to VCAM1. Original magnification 400×. Image is modified, with permission, from REF. 85 © (2001) Oxford Journals.