| Antibodies |
| Rat monoclonal anti-HA (3F10) |
Roche |
Cat#11867431001; RRID:
AB_390919 |
| Rabbit monoclonal anti-Clptm1
(EPR8801) |
Abcam |
Cat#ab174839 |
| Mouse monoclonal anti-Myc (9E10) |
Santa Cruz |
Cat#sc-40; RRID:
AB_627268 |
| Rabbit polyclonal anti-Myc |
Sigma |
Cat#C3956; RRID:
AB_439680 |
| Rabbit polyclonal anti-GFP |
Invitrogen |
Cat#11122; RRID:
AB_221569 |
| Mouse monoclonal
anti-α-Tubulin (DM1A) |
Millipore |
Cat#05-829; RRID:
AB_310035 |
| Mouse monoclonal anti-GM130 |
BD Transduction Laboratories |
Cat#610823; RRID:
AB_398142 |
| Rabbit polyclonal anti-Calnexin |
Enzo Life Sciences |
Cat# ADI-SPA-860; RRID:
AB_10616095 |
| Mouse monoclonal anti-gephyrin
(mAb7a) |
Synaptic Systems |
Cat#147011; RRID:
AB_887717 |
| Rabbit polyclonal
anti-GABAAR γ2 subunit |
(Fritschy et al., 1992) |
N/A |
| Rabbit polyclonal
anti-GABAAR α1 subunit |
Millipore |
Cat#06-868; RRID:
AB_310272 |
| Rabbit polyclonal
anti-GABAAR γ2 subunit |
Synaptic Systems |
Cat#224 003; RRID:
AB_2263066 |
| Mouse monoclonal anti-GAD65 |
Developmental Studies Hybridoma
Bank |
Cat#GAD-6; RRID:
AB_528264 |
| Guinea pig polyclonal anti-vGAT |
Synaptic Systems |
Cat#131 004; RRID:
AB_887873 |
| Chicken polyclonal anti-Map2 |
Abcam |
Cat#ab5392; RRID:
AB_2138153 |
| Rabbit polyclonal
anti-Neuroligin2 |
Synaptic Systems |
Cat#129 203; RRID:
AB_993014 |
| Goat polyclonal anti-GFP biotin
conjugated |
Rockland |
Cat#600-106-215; RRID:
AB_218204 |
| Biological Samples |
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| Chemicals, Peptides, and
Recombinant Proteins |
| n-dodecyl-β-D-maltoside
(DDM) |
Sigma |
Cat#D4641 |
| CNQX |
Abcam |
Cat#ab120044 |
| DL-APV |
Abcam |
Cat#ab120271 |
| Bicuculline methiodide |
Abcam |
Cat#ab120109 |
| Tetrodotoxin (TTX) |
Abcam |
Cat#ab120054 |
| Protein G Sepharose 4 Fast Flow |
GE Healthcare |
Cat#17061801 |
| Protease Inhibitor Cocktail |
Roche |
Cat#05892988001 |
| Ni-NTA Agarose |
Qiagen |
Cat#30230 |
| Streptavidin (Sepharose 4B) |
US Biological |
Cat#S7973-89E |
| Aqua C-18 beads |
Phenomenex |
Cat#00G-4299-N0 |
| Reprosil-Pur C-18-AQ beads |
Dr. Maisch |
Cat#r13.aq |
| C-18 STAGE tips |
(Ishihama et al., 2002) |
N/A |
| TransIT-LT1 Transfection Reagent |
Mirus |
Cat#MIR2300 |
| Critical Commercial
Assays |
| ProFection® Mammalian
Transfection System-Calcium Phosphate |
Promega |
Cat#E1200 |
| Immobilon™ Western
chemiluminescent HRP Substrate |
MIllipore |
Cat#WBKLS0500 |
|
DC™ Protein Assay
Kit |
Bio-Rad |
Cat#5000111 |
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| Deposited Data |
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| Experimental Models: Cell
Lines |
| Human: HEK293 cells |
ATCC |
Cat#CRL-1573 |
| Human: GABAA receptor
α1/β2/γ2 stable cell line HEK293
cells |
ATCC |
Cat#CRL-2029 |
| Monkey: COS7 cells |
ATCC |
Cat#CRL-1651 |
| Rat: embryonic day 18 cortical primary
neuron culture |
This paper |
N/A |
| Rat: embryonic day 18 hippocampal
primary neuron Culture |
This paper |
N/A |
| Experimental Models:
Organisms/Strains |
| Mouse:
Thy1-His6-Flag-YFP-GABAARγ2
(HFY-GABAARγ2) also called
Thy1-YFPγ2 Transgenic Mice |
(Bleckert et al., 2013) |
N/A |
| Mouse: C57BL/6J |
The Jackson Laboratory |
JAX: 000664 |
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| Recombinant DNA |
| Plasmid: Clptm1 |
Open Biosystems |
Cat#MMM1013-649600; GenBank:
BC022172.1
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| Plasmid: Itm2C |
Open Biosystems |
Cat#MMM1013-63801; GenBank:
BC012952.1
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| Plasmid: Glg1 |
Open Biosystems |
Cat#MMM4769-99609980; GenBank:
BC021306.1
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| Plasmid: Tfg |
Open Biosystems |
Cat#MMM1013-202764274;
GeneBank: BC024638.1
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| Plasmid: HA-CD4 |
(Connor
et al., 2016) |
N/A |
| Plasmid: HA-CD8 |
This paper |
N/A |
| Plasmid: Itm2C-Myc |
This paper |
N/A |
| Plasmid: Myc-Glg1 |
This paper |
N/A |
| Plasmid: YFP-Clptm1 |
This paper |
N/A |
| Plasmid: Itm2C-YFP |
This paper |
N/A |
| Plasmid: YFP-Glg1 |
This paper |
N/A |
| Plasmid: YFP-Tfg |
This paper |
N/A |
| Plasmid: Myc-Clstn3-CFP |
(Pettem et al., 2013) |
N/A |
| Plasmid: YFP-Tmub1 |
This paper |
N/A |
| Plasmid:
GABAARα1 |
(Pritchett et al., 1989) |
N/A |
| Plasmid:
GABAARα2 |
(Pritchett et al., 1989) |
N/A |
| Plasmid:
GABAARβ2 |
(Ymer
et al., 1989) |
N/A |
| Plasmid:
GABAARβ3 |
(Ymer
et al., 1989) |
N/A |
| Plasmid:
GABAARγ2 |
(Pritchett et al., 1989) |
N/A |
| Plasmid: GlycineRα1 |
(Liu
et al., 2010) |
N/A |
| Plasmid:
GABAARα5 |
Dharmacon |
Cat#MMM1013-202798742;
GenBank: BC062112.1
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| Plasmid:
HFY-GABAARγ2 |
(Dobie
and Craig, 2011) |
N/A |
| Plasmid:
HA-GABAARα1 |
This paper |
N/A |
| Plasmid:
HA-GABAARα2 |
This paper |
N/A |
| Plasmid:
HA-GABAARβ2 |
This paper |
N/A |
| Plasmid:
HA-GABAARβ3 |
This paper |
N/A |
| Plasmid:
HA-GABAARγ2 |
This paper |
N/A |
| Plasmid: HA-GlycineRα1 |
This paper |
N/A |
| Plasmid: HA-
GABAARα5 |
This paper |
N/A |
| Plasmid: HA-
GABAARα4 |
Sino Biological |
Cat#HG14855-NY |
| Plasmid: HA-
GABAARδ |
Sino Biological |
Cat# HG14909-NY |
| Plasmid: Myc-NT-Clptm1 |
This paper |
N/A |
| Plasmid: Myc-30-Clptm1 |
This paper |
N/A |
| Plasmid: Myc-150-Clptm1 |
This paper |
N/A |
| Plasmid: Myc-382-Clptm1 |
This paper |
N/A |
| Plasmid: Myc-452-Clptm1 |
This paper |
N/A |
| Plasmid: Myc-535-Clptm1 |
This paper |
N/A |
| Plasmid: Myc-CT-Clptm1 |
This paper |
N/A |
| Plasmid: U6-shScramble-hSyn-CFP |
This paper |
N/A |
| Plasmid: U6-shClptm1-hSyn-CFP |
This paper |
N/A |
| Plasmid: Clptm1* |
This paper |
N/A |
| Plasmid:
hSyn-YFP-p2a-Clptm1* |
This paper |
N/A |
| Plasmid:
pAAV-hSyn-YFP-p2a-Clptm1* |
This paper |
N/A |
| Plasmid:
pAAV-U6-shScramble-hSyn-Tdtamato |
This paper |
N/A |
| Plasmid:
pAAV-U6-shClptm1-hSyn-Tdtamato |
This paper |
N/A |
| Sequence-Based
Reagents |
| shRNA targeting sequence: Clptm1 Sh2:
GGCCCTCAACACTTTCATT |
This paper |
N/A |
| shRNA scramble sequence: Sh2 scramble:
GTACACTCACTGTCACTCT |
This paper |
N/A |
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| Software and
Algorithms |
| Pclamp 10.5 |
Molecular Devices |
https://www.moleculardevices.com/systems/conventional-patch-clamp/pclamp-10-software |
| Mini Analysis |
Synaptosoft Inc |
http://www.synaptosoft.com/MiniAnalysis/ |
| Image J |
National Institute of Health |
https://imagej.nih.gov/ij/index.html |
| GraphPad Prism |
GraphPad Software Inc |
http://www.graphpad.com/scientific-software/prism/ |
| Image Lab |
Bio-Rad |
http://www.bio-rad.com/en-ca/product/image-lab-software |
| Proteome Discoverer v. 1.2 |
ThermoFisher Scientific |
https://www.thermofisher.com/order/catalog/product/IQLAAEGABSFAKJMAUH |
| Over-representation analysis |
Geneontology |
http://pantherdb.org/geneListAnalysis.do |
| Uniprot |
Uniprot |
http://www.uniprot.org/ |
| Tmpred |
Omic Tools |
https://omictools.com/tmpred-tool |
| Other |
