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. Author manuscript; available in PMC: 2019 Feb 8.
Published in final edited form as: Cell. 2018 Feb 8;172(4):683–695.e15. doi: 10.1016/j.cell.2018.01.005

Figure 5. Loss of striatal FSIs impairs sequence learning in naive animals but does not affect performance in expert mice.

Figure 5

(A) Task design for 3-port sequence task. (B,C) Ablating striatal FSIs with AAV-DIO-Casp3 reduces accuracy early but not late in learning (N=11 mice per group; two-way ANOVA PGroup<10−5; unpaired two-tailed t-test P<0.05 early; P>0.05 late). (D) FSI ablation does not affect motor performance (N=11 mice per group; two-way ANOVA PGroup>0.05). (E) Mice were pre-trained untethered for at least 5 days, followed by 1–2 days of habituation to optical fibers before experiencing two laser stimulation sessions. (F) Optogenetically suppressing FSIs on interleaved trials in expert mice does not affect error rate (paired two-tailed t-test P>0.05), (G) efficiency (sign rank test P>0.05) or (H) motivation in sequence task (paired two-tailed t-test P>0.05; N=9 mice per group all conditions). Optogenetic silencing for full trial duration (see methods). All error bars are s.e.m. and center values are group means.