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. 2016 Nov 16;11(5-6):447–463. doi: 10.1080/19336918.2016.1247144

Figure 2.

Figure 2.

A-type lamin depletion reduces FA and rescue of LMNA expression alleviates the phenotype. (A) Representative images of NTkd and LMNAkd cells after immunofluorescence staining for paxillin, vinculin or FAK (yellow) and counterstaining with DAPI (cyan). (B) Normalized mean fluorescence (%) of FA points relative to the average of NTkd. (C) Quantification of the number of FAs per cell. (D) Area per FA (µm2). (E) Western blot for paxillin, vinculin and FAK. Nucleolin or actin was used as a loading control. (F) Number of FAs per cell relative to NTkd control 1 week after LMNAkd was either arrested or continued. (G) FA area relative to NTkd control cells. (H) Lamin A level relative to NTkd control cells. (I) Normalized mean fluorescence (%) of nuclear FAK relative to NTkd cells. Immunofluorescent staining was performed in triplicate and a minimum of 100 cells were imaged per replicate. In the boxplots the horizontal line indicates the median, boxes the 25th and 75th percentile, and whiskers the 5th and 95th percentile. For all figures, significance was calculated with the Wilcoxon rank sum test; *** = p-value < 0.001.