Table 3.
PCR and pyrosequencing primer sequences.
| Gene name | Gene region | Primer | Sequence |
|---|---|---|---|
| Bdnf | Promoter IV | Forward primer (5' to 3') | TTTTTAGTTTTTGTTTAGATTAAATGGAGT |
| Reverse biotin primer (5' to 3') | CAACAAAAAAATTAAATTATTAATAATAAA | ||
| Sequencing primer (5' to 3') | GTTTTTTATTGAAGG | ||
| Sequence analyzed | C/TGTGC/TGAGTATTATTTTC/TGTTATG | ||
| Comt | Promoter P1 | Forward primer (5' to 3') | GTTTGTTGTAGTTTGGAGTTAGGT |
| Reverse biotin primer (5' to 3') | CTCATCCTCCCCCATTACCT | ||
| Sequencing primer (5' to 3') | TATGGTTTAGTGTGG | ||
| Sequence analyzed | C/TGGTTGTGGGGTGTAGGGGGC/TGGGGGGGT | ||
| Nr3c1 | GR110 | Forward primer (5' to 3') | GGTTGGTAAAAGTTTGTTAAGTT |
| Reverse biotin primer (5' to 3') | CTAAAAACTCTCCCCTCCCC | ||
| Sequencing primer (5' to 3') | GTTTATTTTAGTATT | ||
| Sequence analyzed | C/TGC/TGC/TGTTC/TGTTC/TGTTC/TGC | ||
| Il1b | Promoter | Forward primer (5' to 3') | TGTATAAGGAAGTTTGATTGGAGAG |
| Reverse biotin primer (5' to 3') | ATAAAATCAATTAACCCAAAAAAAA | ||
| Sequencing primer (5' to 3') | ATGTTTTGAATTATT | ||
| Sequence analyzed | C/TGGGGTTTGCTGTCCACTAGTTTTCTCTCCCTC/TGTTTTA | ||
| Il6-1 | Promoter | Forward primer (5' to 3') | TTGTGATTTTTTGGATGTTAAATGA |
| Reverse biotin primer (5' to 3') | CAAACATCCCCAATCTCATATTTAT | ||
| Sequencing primer (5' to 3') | TTGTGATTTTTTGGATGTTAAATGA | ||
| Sequence analyzed | C/TGTTATAT | ||
| Il6-2 | Promoter | Forward primer (5' to 3') | TTGTGATTTTTTGGATGTTAAATGA |
| Reverse biotin primer (5' to 3') | CAAACATCCCCAATCTCATATTTAT | ||
| Sequencing primer (5' to 3') | TTAAAAGTAGAGAGT | ||
| Sequence analyzed | C/TGATTTTTA | ||
| Tnf | Promoter | Forward primer (5' to 3') | GGATTGTTATAGAATTTTGGTGAGG |
| Reverse biotin primer (5' to 3') | ACTTCCTTAATAAAAAAAACCATAATCTC | ||
| Sequencing primer (5' to 3') | TTAAATTTTTGTTTT | ||
| Sequence analyzed | C/TGTATTGGAGAAGAAATTGA | ||
| MT-co1 | Exon | Forward primer (5' to 3') | AGTTGGAGTTGGAATAGGATGAATA |
| Reverse biotin primer (5' to 3') | TAACTCCTAAAATAAAAAACACCCC | ||
| Sequencing primer (5' to 3') | ATATTTTTTTTTAGT | ||
| Sequence analyzed | C/TGGAAATTTAGTTTATGTTGGGG/ATATTC/TGTAGATTTAA | ||
| MT-co2 | Exon | Forward primer (5' to 3') | TAATGATTTAAAATTAGGTGAATTT |
| Reverse biotin primer (5' to 3') | TAACCCTAATAAAAAAATAACTCATAAATA | ||
| Sequencing primer (5' to 3') | ATAGAATTTTTAATT | ||
| Sequence analyzed | C/TGTATATTAATTTTATTC/TGAAGAC/TGTTTTG | ||
| MT-co3 | Exon | Forward primer (5' to 3') | GTTATTATATTTTTATTGTATAAAAAGGTT |
| Reverse biotin primer (5' to 3') | AAATAATAAAATACTCAAAAAAATCC | ||
| Sequencing primer (5' to 3') | GTATAAAAAGGTTTT | ||
| Sequence analyzed | C/TGATAC/TGGAATAATTTTGTTTATTGTTTTC/TGAAGT | ||
| L1-utr | - | Forward primer (5' to 3') | GGTGTATAGGTTTTTTTGGTTGTTG |
| Reverse biotin primer (5' to 3') | AAATTCACCAAACAACTTTCTTACAA | ||
| Sequencing primer (5' to 3') | TTTTTTTGGTTGTTGT | ||
| Sequence analyzed | C/TGTTGTAGAGAGTTC/TGTGGTAGTATTTTA | ||
| L1-orf | - | Forward primer (5' to 3') | AGAAAGAATATTAAAAATAGTAAGGGAAAA |
| Reverse biotin primer (5' to 3') | ATCAATCCAAAATCTTCTAACCTTC | ||
| Sequencing primer (5' to 3') | TTATATTAGATTTTT | ||
| Sequence analyzed | C/TGTTAGAAATTAT |