Figure 5.

Knock-out of GPRC5A modulates the activity of RhoA and Rac1 GTPases. (A) Western blot demonstrating the amount of RhoA, Rac1, and cdc42 GTPases immunoprecipitated in GTP-bound state from the cells collected 30 minutes after plating on Collagen I (0.1 mg/mL). Three control (C1-3) and 3 knock-out (K1-3) samples were collected independently (N = 3). (B) Quantification of the experiment shown in (A). The signal from precipitated GTP-bound GTPases was normalized to the total amount of a corresponding GTPase protein and the loading control, and presented as % of control. Bars represent mean ± SEM, N = 3. Statistical significance was evaluated using one-way ANOVA test. *, p < 0 .05.