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. 2017 Feb 6;11(5-6):434–446. doi: 10.1080/19336918.2016.1245264

Figure 6.

Figure 6.

GPRC5A interacts with EphA2 tyrosine kinase. MDA-MB-231 cells were cultured on Collagen I – coated surface for 48 h. Proteins from MDA-MB-231 control (Ctrl) or GPRC5A knockout (KO) cells were immunoprecipitated. (IP) using either rabbit anti-GPRC5A polyclonal antibodies (G5A), or rabbit non-immune IgG (IgG) as a control as described in Materials and Methods. Both protein lysates and eluted protein complexes were separated on the same SDS-PAGE gel and analyzed by Western blotting (WB).