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(a) Fluorescence intensity per nucleotide with respect to oligomer lengths. Fluorescence of polynucleotides with 1-, 2-, 4-, 8-, 16- and 20-base were excited at 500 nm. Comparison of (b) fluorescence intensity per nucleotide and (c) Molar extinction coefficient per nucleotide for mononucleotide and 20-base polynucleotide. Critically, this indicates a non-linear relation between sequence length, fluorescence intensity, and excitation coefficient.
