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. Author manuscript; available in PMC: 2018 Feb 13.
Published in final edited form as: Dev Biol. 2016 Apr 20;415(1):111–121. doi: 10.1016/j.ydbio.2016.04.004

Figure 1. Four types of extravascular islands.

Figure 1

(A) Schematic illustrating the cell types identified by the combination of markers used in the analysis. (B) The four types of extravascular islands. Type I consists of tightly arranged hematopoietic cells with no endothelial component. Type II islands contain tightly arranged hematopoietic cells wrapped in a single layer of endothelial cells. Type III has loosely arranged hematopoietic cells wrapped in an endothelial layer. Type IV consists of a sphere of endothelial cells that are not associated with hematopoietic cells. (C) Z-projection of a type IV extravascular island that has been immunostained for CD31 (i,ii), Runx1 (i,iii) and Kit (i,iv). Panel on the right is a single optical projection illustrating the lack of Runx1+ Kit+ hematopoietic cells within a type IV extravascular island. Z-interval = 2μm and scale bar = 50μm.