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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Cancer Res. 2017 Nov 8;78(1):143–156. doi: 10.1158/0008-5472.CAN-17-0240

Figure 5. Silencing C3a Receptor Expression in LLC-luc Cells Inhibits the Primary Tumor Growth, but not metastasis in vivo.

Figure 5

(A) Flow cytometric analysis of cell surface C3a receptor expression on the two C3a receptor knockdown and control LLC-luc cell lines. (Isotype in light grey (median = 9.10); LLC-luc shRNA control on grey (median = 19.8); LLC-luc C3aR KD#1 in dark grey with small dotted line (median = 9.87); LLC-luc C3aR KD#2 in dark grey with big dotted line (median = 10.5)). (B) Fold changes in vitro proliferation of two C3aR knockdown LLC-luc cell lines are shown. (C and D) Numbers and sizes of C3aR KD LLC-luc colonies are shown in the anchorage independent assay. (E-G) The primary tumor volumes (E) and number of pulmonary (F) and liver (G) metastases induced by the two C3aR KD LLC-luc cell lines and the control shRNA cell lines are shown. The animals were sacrificed 21 days after the tumor implantation. (WT+C3aR KD#1 n = 5; C3−/−+C3aR KD#1 n = 5; WT+C3aR KD#2 n = 5; C3−/−+C3aR KD#2 n =4). * p<0.05 Error bars represent mean ± SEM.