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. Author manuscript; available in PMC: 2018 Feb 13.
Published in final edited form as: Cell Rep. 2018 Jan 28;22(4):869–875. doi: 10.1016/j.celrep.2017.12.082

Figure 2. Transfer of Human SERINC5 ICL4 Is Sufficient to Confer Nef Responsiveness to Frog SERINC5.

Figure 2

(A) Frog SERINC5 is as potent as human SERINC5 in inhibiting the single-round infectivity of Nef− HIV-1 progeny virions.

(B) Schematic illustration of the parental SERINC5 proteins and of the chimera examined.

(C) Effect of frog SERINC5 on Nef− HIV-1 progeny virion infectivity is largely resistant to NefSF2 but becomes sensitive upon replacement of its ICL4 by that of human SERINC5. SERINCs were expressed from pBJ5 or from the very weak pBJ6 expression plasmid.

(D) Western blots showing the effects of NefSF2 on the incorporation of human, frog, and chimeric SERINC5 into Nef− HIV-1 virions.

Bar graphs represent the mean + SD from 3 biological replicates.

See also Figures S1, S2, and S4.