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. Author manuscript; available in PMC: 2018 Feb 13.
Published in final edited form as: Cell Rep. 2018 Jan 28;22(4):869–875. doi: 10.1016/j.celrep.2017.12.082

Figure 4. ICL4 of Human SERINC2 Confers Resistance to NefSF2, but Not Nef97ZA012.

Figure 4

(A) Schematic illustration of the parental human SERINC proteins and of the chimera examined.

(B) Inhibitory activity of human SERINC5 bearing the ICL4 of human SERINC2 on Nef− HIV-1 progeny virion infectivity is largely resistant to NefSF2. Bar graphs represent the mean + SD from 3 biological replicates.

(C) Western blots showing that the incorporation of the SERINC chimera into Nef− HIV-1 virions is largely unaffected by NefSF2 expressed in trans. The incorporation of the chimera was analyzed in duplicate to document reproducibility.

(D) Western blots showing that the incorporation of human SERINC2 into Nef− HIV-1 virions is unaffected by NefSF2 but inhibited by Nef97ZA012.

(E) Western blots showing that Nef97ZA012 inhibits the incorporation of human SERINC5 bearing the ICL4 of human SERINC2 into Nef− HIV-1 virions.

See also Figures S3 and S4.