Fig. 4.

MDCC-SSB applied to study the impact of myosin VI motor activity upon RNA Polymerase II transcription. (A) In vitro transcription by HelaScribe extracts. Reactions were performed under standard conditions and following antibody depletion, or in the presence of 25 μM TIP myosin VI inhibitor, as described in the Materials and Methods. MDCC-SSB was added to the samples and the fluorescence enhancement was determined under the different conditions. For RT-qPCR, samples were normalized to a non-depleted control reaction (error bars represent SEM). (B) Changes in gene expression following the addition of TIP to MCF-7 cells. Expression is plotted as a percentage of expression in mock cells (Error bars represent SEM).