Figure 1.

A) Results of T-ARMS-PCR of eNOS polymorphism (rs1799983). F1 and R1: outer primers for control PCR product (701 bp); F2 and R2: inner primers for two allele-specific PCR products G allele (475bp) and T allele (271bp). GG genotype results 2 bands (701 and 475 bp) and TT genotype results 2 bands (701 and 271 bp) and 3 bands in GT genotype (701, 475 and 271bp). B) Validation using PCR-RFLP by MboI restriction endonuclease