Table 1. PCR primers and conditions. F1 and R1 (outer primers), F2 and R2 (inner primers) were designed for T-ARMSPCR. To enhance the specificity of inner primers (F2 and R2), at the 3rd nucleotide from the 3´-terminus changes to destabilizing mismatch (the bold underlined bases indicate the extra mismatched bases introduced). F3 and R3 primers were used for PCR- RFLP reaction.

Primer sequence	Tm (ºC)	Amplicon size (bp)
F1: 5'-AGCCTCGGTGAGATAAAGGATG-3' R1: 5'-CCTGGACCTGCTCTGATTGTC-3' F2: 5'-GCTGCTGCAGGCCCCAGATAAG-3' R2:5'-GCAGAAGGAAGAGTTCTGGGAGA-3' F3: 5'-TCACGGAGACCCAGCCAATGAG-3' R3: 5'-TCCATCCCACCCAGTCAATCCC-3'	66 66 72 70 70 70	G allele (475) T allele (271) Control band (701) G allele undigested (293) T allele digest with MboI (197+95)