Table 2. Benefit and cost details in use to T-ARMS-PCR and PCR-RFLP methods for genotyping the eNOS rs1799983 polymorphism.

Method	T-ARMS-PCR	PCR-RFLP
Materials for reaction	● Enzyme and buff ers in PCR	● Enzyme and buff ers in PCR Restriction enzyme and buffers (MboI enzyme)
Time	● 2.5 h for PCR	● 1.5 h for PCR 3 h for RFLP reaction
Cost	● Cost 3.00 US Dollar per sample	● Cost 4.00 US Dollar per sample
Problems	● Fast, no digestion problems ● Common agarose gel electrophoresis ● No restriction enzyme ● No digestion	● Lengthy, incomplete digestion problems ● High resolution agarose gel electrophoresis ● MboI restriction enzyme ● Digestion for 3 h