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. 2018 Feb 1;14(2):e1006843. doi: 10.1371/journal.ppat.1006843

Fig 5. Accelerated gene expression coupled with replication defect upon 75A.stop infection of primary cells.

Fig 5

(A) Single-step growth curve in primary BMDM at an MOI of 5 with 75A.stop1.2 and 75A.stop1MR. (B) Timecourse analysis of gene products upon a single-step infection of BMDMs. (C) Multi-step growth curve in primary MEFs at an MOI of 0.05 with 75A.stop1.2 and 75A.stop1MR. (D) Timecourse analysis of gene products upon a low MOI infection of MEFs. (E) Schematic of the ORF75 locus and the multicistronic transcripts with strand-specific probes to indicated ORFs. (F) Northern blot analysis of NIH 3T12 fibroblast cells infected with indicated viruses at an MOI of 5. Membrane was hybridized with strand-specific 32P-labled cDNA of ORF75A, ORF75B, ORF75C, M3, or 18S. Longer exposures with ORF75C probe detects the orf75A/B/C transcript. (G) Viral DNA quantification of input genomes at 2 hpi and subsequent replication at 24 hpi. For A, C, and E, bars represent the mean of three independent biological replicates +/- SD.