Fig. 2. (a) The labelling of SPIN1 by probes 1, 2 and 3. Recombinant SPIN1 (20 ng μL^–1) was incubated with probes 1, 2, and 3 (2 μM), respectively. After UV irradiation (365 nm) for 1 h, the labelled SPIN1 was conjugated to a rhodamine-azide tag, resolved by SDS-PAGE, and detected by in-gel fluorescence scanning. (b) Labelling yields of SPIN1 with probes 1, 2, and 3 (2 μM) during the course of UV irradiation (365 nm). Labelling yields of (c) MORC3 and (d) ING2 with probes 1, 2, and 3 (2 μM), respectively. Data are averages ± s.e. (n = 3).