Fig. 2. Solution speciation of PyBD (50 μM). Left: UV-vis spectra in the range of pH 2–8. Right: solution speciation diagram (F L = fraction of compound in given protonation state). Bottom: acidity constants of L (L = PyBD) with charges omitted for clarity. Speciation was performed at room temperature with I = 0.1 M NaCl. aError in the last digit is indicated in parentheses.
