Fig. 4. Reactivity of PyED and PyBD with preformed Aβ40 aggregates. (A) Analysis of resultant Aβ40 species by gel electrophoresis with Western blot using an anti-Aβ antibody (6E10). TEM images of the samples incubated for 24 h at (B) 37 °C or (C) 43 °C. Experimental conditions: [Aβ] = 25 μM; [CuCl2 or ZnCl2] = 25 μM; [PyED or PyBD] = 50 μM; 2, 8, 24 h incubation at 37 or 43 °C; pH 7.4 (metal-free and Zn(ii)) or pH 6.6 (Cu(ii)); constant agitation.
