Fig. 4. Expansion of positional encoding capacity through an increase in longitudinal dimension. (A) Schematic illustration of DCU design and two-target identification at discrete locations of both cuboids. (B) TEM image of DCU and DUT1 in the presence of target DNA TT1. (C) TEM image of DCU and DUB3 in the presence of target DNA TB3. (D) TEM image of DCU, DUT1, and DUB3 in the presence of targets TT1 and TB3 (hybridization was performed separately for DCU and DU for each target and then the hybridization solutions were combined into a single sample for TEM imaging). (E) TEM image of DCU, DUT1, and DUB3 in the presence of targets TT1 and TB3 (hybridization was performed simultaneously for DCU and DU for two targets and then the sample was subjected to TEM imaging).
