Fig. 3. (A) Fluorescence intensity responses of the double-mismatch/fluorophore modified DNA probe (P-3FAM-4T) in the detection of different SNPs at position +I; (B) fluorescence intensity responses of P-3FAM-4C in the detection of DNA point mutations at different abundances. The inset shows the mean values and standard deviations of the rate of fluorescence increase over the time period from 100 s to 600 s for the background solution and the two tested solutions with the lowest abundances (i.e. 0.05% and 0.2%). The probe sequence is 5′PO4-TCT(-FAM)NCACAGACACATACTCCA-BHQ1. The target sequence is 5′GTTTTAAATTATGGAGTATGTGTCTGTTMAAACGA-GAGTAAG. 100% means the tested strands are all mutant type (N:M = C:G). 0% means the tested strands are all wild-type (N:M = C:C).
