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. Author manuscript; available in PMC: 2018 Feb 13.
Published in final edited form as: J Proteome Res. 2016 Aug 29;15(10):3813–3826. doi: 10.1021/acs.jproteome.6b00602

Figure 2.

Figure 2

HTS treatment priming of increases and nuclear translocation of p53 and preservation of the stability of the p53 pool through promotion of degradative phosphorylation at T155. Fluorescence microscopy images indicate that the total levels of cytosolic p53 and nuclear p53 increase after treatment with HTS and HTS+CMX (A–D,E). However, CMX treatment (either alone or in combination with HTS) decreased the percentage of cytosolic p53 phosphorylated at T155 (F–I), a post-translational modification that primes p53 degradation (J). Labeling: p53 (A–D) or pT155-p53 (F–I, red), cell nuclei (DAPI, blue), and membranes (WGA, green).