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. 2018 Jan 22;7:e33116. doi: 10.7554/eLife.33116

Figure 2. Identification of two new Dsc components.

(A) A cartoon model showing known Dsc components in budding yeast. (B) A silver staining gel showing that Ypr109W and Yir014W co-immunoprecipitate with Ubx3-Flag, together with other known Dsc components. HC, heavy chain. LC, light chain. (C) 014W-Flag can selectively pull down known Dsc components. (D) 014W-Flag can selectively pull down with known Dsc components. (E) Cartoon representations of 014W and 109W. Both proteins are predicted to be tetra-spanning membrane proteins. (F) 014W-HA and 109W-Flag do not pull down each other. St: Starting material. Ub: Unbound. El: Elution.

Figure 2.

Figure 2—figure supplement 1. Identification of two new Dsc subunits by mass spectrometry.

Figure 2—figure supplement 1.

(A) Mass spectrometry peptide coverage for Yir014W. The peptides identified by mass spectrometry are highlighted in red. The underlined sequences are the predicted transmembrane helices. (B) Mass spectrometry peptide coverage for Ypr109W. The peptides identified by mass spectrometry are highlighted in red. The underlined sequences are the predicted transmembrane helices. (C) Sequence similarity between Yir014W and Ypr109W. The alignment was performed with Clustal Omega (Sievers et al., 2011).