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. 2018 Jan 22;7:e33116. doi: 10.7554/eLife.33116

Figure 3. 109W and 014W have distinct subcellular localizations.

Figure 3.

(A) 014W-nG co-localizes with FM4-64 labelled vacuole membrane, whereas 109W-nG localizes to the intracellular punctae. (B) Quantification of the number of 109 W-nG punctae. (C) Left, 109W-nG partially co-localizes with Mars-Sec7 labelled trans-Golgi (white arrows). Right, quantification of the co-localization. Error bar represents the Standard Error of the Mean (SEM). (D) Left, 109W-nG partially co-localizes with FM4-64 labelled endosomes (white arrows). Right, quantification of the co-localization. Error bar represents the SEM. (E) Co-localization between 109W and Ubx3 using rapamycin induced co-localization (RICo) assay. Left: A cartoon diagram showing the principle of the assay. Right: FRB-mCherry localization before and after rapamycin treatment. White arrows indicate the co-localization. Scale bar: 2 µm.

Figure 3—source data 1. The source data for the quantification of 109W-nG punctae in Figure 3B.
elife-33116-fig3-data1.xlsx (12.1KB, xlsx)
DOI: 10.7554/eLife.33116.007
Figure 3—source data 2. The source data for the quantification of co-localization in Figure 3C-D.
elife-33116-fig3-data2.xlsx (16.9KB, xlsx)
DOI: 10.7554/eLife.33116.008